Fluorescence in Situ Hybridization & Illumination Light Sources
Fluorescence In Situ Hybridization is a method that uses fluorescence to label nucleotides (DNA and RNA). This method allows for researchers to image and locate proteins that have been expressed/transcribed (as DNA or RNA), providing spatio-temporal information about gene expression within cells and tissues. FISH signals can sometimes be low, depending on the protocol employed for staining the sample. As there are multiple fluorophores to be imaged in FISH, narrow-band filters are often used to enable signal separation, thereby reducing excitation light to the sample. Thus, between potentially low signals and narrow-band filter use, a powerful illumination source is required to excite fluorescence in the sample.
An X-Cite® 120Q or X-Cite XYLIS illumination system enables easy viewing of such challenging samples owing to powerful light intensity when compared with traditional light sources.