Live Cell Imaging with X-Cite
Live Cell Mode®
As the name indicates, live cell imaging involves studying live cells (mostly cultured cells) or tissue through various fluorescence microscopy techniques. These cells are made to fluorescently express the protein of interest (via expression, e.g. GFP, YFP, or staining with
DAPI, DRAQ, pH and Ca++ probes). Studying cells in this manner is an excellent tool to monitor protein trafficking, signal tranduction and protein interactions. The biggest challenges in this technique include reducing the photobleaching effects on fluorophores and minimizing the phototoxic effects of light on sample viability. The X-Cite solutions enable you to control lamp intensity to best suit your experimental setup.
Light delivery via a liquid light guide, and effective cooling prevents vibrational artifacts and experimental interruptions caused by these factors. Additionally, the light can be shuttered in order to reduce sample exposure when the cells are not being imaged. The X-Cite XYLIS
broadband light source offers a feature which allows the UV part of the spectrum to be turned OFF in order to increase sample viability.
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» View Is Pulsed LED Illumination Less Damaging to Living Cells? (PDF)
» View Use of White Light Fluorescence LED in Long-term Live Cell Imaging Applications (PDF)
» View Pulsed LED Illumination Reduces Phototoxicity in Living Cell (PDF)
For more advanced applications, we have shown that using the X-Cite XLED1 in
Live Cell Mode® or pulsed mode leads to lower phototoxicity in live cells
(Live Cell poster)
when compared to continuous light exposure. This could either be due
to the ability of the cell to recover from being constantly exposed to
light thereby generating fewer ROS (reactive oxygen species) or due to
the fact that the fluorophore molecules have a chance to relax to ground
state with fewer molecules remaining in the triplet state, allowing the
fluorophore to be excited more efficiently with less light exposure.
For white light broadband system that provides the illumination
uniformity and stability required for Live Cell Imaging, our users
tested the X-Cite 120LED on a two-day time lapse imaging experiment.
The experiment can be seen here.
The X-Cite 120LED
was more uniform and stable when compared to their HBO lamp, and also
allowed the cells to proliferate marginally more than cells imaged with
an HBO lamp over the same duration.
X-Cite will assist in your live cell imaging & fluorescence microscopy through:
- Reduced photobleaching
- Reduced phototoxicity
- Uniform field of view
- Vibration-free experiments
- Happy Cells – better viability when compared with a traditional HBO lamp during Time-lapse Imaging